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Thermo Scientific Varioskan Lux Multimodal Microplate Reader

  • fast and reliable results
  • automatic dynamic range selection
  • built-in safety checks.

Original price was: $19,999.00.Current price is: $14,999.00.

SKU:Varioskan LUX

Varioskan LUX Microplate Reader offers automatic dynamic range selection, which adjusts the optimal reading range based on signal intensity, and built-in safety checks alerting you to potential errors.

The Varioskan LUX multimodal microplate reader is designed specifically for life science researchers to provide fast and reliable results for even the most challenging applications.

Thermo Fisher Scientific’s new Varioskan LUX comes with different measurement technologies, including absorption and fluorescence intensity with optional Luminescence, AlphaScreen, and a Time-Resolved fluorescence module. The Varioskan LUX can also be equipped with up to two onboard dispensers, allowing for the easy and accurate addition of reagents.

Among other features, the equipment also has agitation, an integrated gas control module, automatic dynamic range selection, and intelligent safety controls that detect errors before they happen. This gives researchers confidence that their experiment will run smoothly and successfully.

With the Varioskan Lux, it is possible to use plates from 6 to 1,536 wells and be compatible with the µdrop plate for micro volumes.


Key Features

  • Varioskan LUX is a spectral scanning flatbed fluorometer with spectrophotometry, fluorescence (including TR-FRET, hTRF), Luminescence (including BRET), and AlphaScreen/AlphaLISA functions.
  • The measurement areas of this design are absorbance and fluorescence. Absorption wavelength range 200 – 1000 nm. The design includes a built-in gas module to control the concentration of CO2 and O2 for cell analysis, a shaker, an incubator, 1and a dispenser.
  • For precise dosing of reagents, the Varioskan LUX can be equipped with onboard dispensers, which guarantees reproducible results and dosing accuracy.
  • The built-in incubator ensures an optimal and stable temperature in the reaction medium.
  • Varioskan LUX is easily integrated with automated systems. The device is designed in either case the robotic station can work with microplates in the “portrait” or “landscape” mode.
  • Thermo Scientific Skanlt software with automatic calibration and self-diagnosis avoids errors. Based on the intensity of the signal, the dynamic range is adjusted automatically—availability of interface for integration with automated systems.

The Varioskan LUX is a modular, upgradable instrument that provides excellent usability across various applications.

  • Up to five measurement technologies: Absorbance (UV-Vis), Fluorescence intensity (incl. FRET), Luminescence (direct and filtered), AlphaScreen/AlphaLISA, and Time-resolved fluorescence (incl. TR-FRET/hTRF)
  • Filter and monochromator optics
  • Spectral scanning for assay optimization
  • Five measurement modes, including endpoint, kinetic, spectra, kinetic spectra, and multipoint
  • Integrated gas control module for setting CO2 and O2 concentrations for cell-based assays
  • Simultaneous dispensing and measurement for signal monitoring at the start of a reaction
  • Automatic dynamic range selection, based on the signal intensity of each well
  • Smart safety controls are designed to identify and alert the user of potential problems before they happen.
  • Automated instrument calibration and self-diagnostics for reliable performance
  • Certified HTRF compatible reader by CisBio Bioassays
  • Validated DLReady by Promega Corporation

Varioskan LUX is paired with the powerful and intuitive SkanIt Software, providing excellent usability and flexibility.

  • License free—Each user can install the software on their computer
  • An intuitive and flexible interface for multiple users and assays
  • Innovative Virtual Pipetting Tool makes it easy to define samples to plate layout
  • Measurement data is continuously saved to the database, in either case there’s no data lost due to unexpected interruptions such as power outages or accidental aborting
  • Simplified data processing and export
    • Export data in a single mouse click, or have it emailed automatically at the end of the run
    • .Several file formats for exporting results: *.xlsx, *.pdf, *.xml, and *.txt
  • Shows real-time spectra and kinetic curves during the measurement
  • Built-in calculations for data analysis (e.g., blank subtraction, average/SD/CV%, standard curve, dose-response, quality control, classification, kinetic and spectral analysis, custom formula, etc.)
  • Comprehensive result reports with traceability to runtime actions and settings
  • Two editions are available: Research Edition for life science researchers and Drug Discovery Edition, which offers features needed for the FDA 21 CFR Part 11 compliance.
The Varioskan LUX is modular and upgradeable, in either case you can build the system that best suits your needs. For more information, contact a sales representative.

Both software can be operated in nine languages ​​(English, French, German, Spanish, Portuguese, Italian, Chinese, Japanese, and Russian).


What makes Varioskan LUX great product:

  • Built-in intelligence: from the intelligent safety controls, automatic dynamic range selection,and auto-calibration, Varioskan LUX determines the ideal settings to start an experiment
  • Excellent interface: the SkanIt Software is highly intuitive o which ensures the equipment is simple to operate even for the most complex tests
  • Flexibility: Varioskan LUX is highly configurable with a wide range of features that can be added to meet customer needs for a wide range of applications


  • 2016 Thermo Varioskan LUX Multimode Microplate Reader | CAT# VLB00D0
  • Laptop Running SkanIt Software
  • Absorbance Validation Report
  • Fluorescence Validation Report
  • Absorbance Module Installed
  • Fluorescence Module Installed (Top/Bottom)
  • Luminescence Module Installed
  • Communication Cable
  • 120V Power Cord
  • User Manual
  • 90-Day Warranty

Reference Link

  • CompatibilityRobot
    Detector TypePhotomultiplier Tube (PMT)
    Incubator TemperatureUp to 45°C
    InterfacePC Software (Thermo Scientific SkanIt Software)
    Light SourceXenon Flash Lamp
    Measurement Speed15 s for 96-well Plate, 45 s for 384-well Plate, 135 s for 1536-Well Plate (Minimum Times)
    Measurement TechnologyAbsorbance, Fluorescence Intensity, Luminescence
    Plate Type6 to 1536-well Plate (Absorbance 6 to 384-well Plate)
    Product LineVarioskan™
    Reading OptionsTop and Bottom
    Read-out Range0 to 6 Abs
    Software TypeSkanIt Software
    Spectral Scanning Speed<2.2 s/Well 400 to 500 nm, 1 Flash, 2 nm Step
    Wavelength SelectionAbsorbance & Spectral Scanning: Double Monochromator, Fluorescence Intensity: Double Excitation and Emission Monochromator
    Accuracy0.003 Abs or ±2%, at 200 to 399 nm (0 to 2 Abs)
    0.003 Abs or ±1%, at 400 to 1000 nm (0 to 3 Abs)
    Depth (English)23 in.
    Depth (Metric)58 cm
    Dynamic RangeFluorescence intensity: top reading > 6 decades, bottom reading > 5.5 decades
    Luminescence: > 7 decades
    Height (English)20 in.
    Height (Metric)51 cm
    Hertz50/60 Hz
    Linearity96-well plate: ±2% (0 to 4 Abs at 450 nm)
    384-well plate: ±2% (0 to 3 Abs at 450 nm)
    Measurement TypeEndpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra
    No. of DispensersNone
    PrecisionSD < 0.001 Abs or CV < 0.5%, at 450 nm (0 to 3 Abs)
    SensitivityFluorescence intensity (top reading): < 0.4 fmol fluorescein/well (black 384-well plate)
    Fluorescence intensity (bottom reading): < 4 fmol fluorescein/well, (clear bottom black 384 square well plate)
    Luminescence: < 7 amol ATP/well (white 384-well plate)
    Software Language VersionsEnglish, German, French, Spanish, Portuguese, Italian, Japansese, Chinese and Russian
    Voltage100/240 V
    Wavelength RangeAbsorbance: 200 to 1000 nm
    Fluorescence intensity: Excitation: 200 to 1000 nm, Emission: 270 to 840 nm
    Weight (English)123 lb.
    Weight (Metric)56 kg
    Width (English)21 in.
    Width (Metric)53 cm
    Unit SizeEach


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