Name: BMG LabTech CLARIOstar Plus Multi-Mode Plate Reader
Brand: BMG Labtech
SKU: CLARIOstar Plus
Price: 19999 USD
Availability: InStock
Rating: 5 (5 reviews)

CLARIOstar Plus is a modularly expandable high-performance microplate reader that meets all laboratories’ current and future requirements. The device measures the leading detection technologies, including UV/vis absorbance, fluorescence intensity (incl. FRET), luminescence (incl. BRET), fluorescence polarization, time-resolved fluorescence (incl. TR-FRET), and AlphaScreen.

“I am using the clariostar plus plate reader for luminescence-, fluorescence-, FRET- and BRET-based assays. The plate readers shows a very good performance in all these modes and the user-friendly, intuitive control surface facilitates the fast setup of new experiments. The analysis software MARS enables a fast analysis and export of the raw data. What would improve the performance of the plate reader in dual-color assays (FRET & BRET), is the design of a filter cube that would allow for the simultaneous detection of both channels (as feasible with, e.g., the Neo2 plate from BioTek).”
Hannes Schihada – Philipps-University Marburg

CLARIOstar® multimodal reader, unlike readers equipped with classic monochromators, allows you to change the transmission slit width from 8 to 100 nm, significantly increasing its sensitivity. The presence of a library of fluorophores built into the software greatly simplifies the workflow.

Features

Innovative monochromators for increased sensitivity
Fully-flexible wavelengths and bandwidths
Spectral scanning in absorbance, fluorescence intensity, and luminescence
Largest dynamic range with no manual intervention
Rapid full-plate autofocus
Dedicated features for live cell-based assays
Ultra-fast data sampling (100 measurements/second)
Dedicated laser for Alpha Technology
Easily integrated into automated systems

Advantages

It’s all on board: monochromator, filters, and spectrometer for maximum flexibility and sensitivity.
Simplified measurement setup: widest concentration and signal intensity range, auto-focus function
Optimized for long-term measurements with living cells: easy control of O2, CO2, de-, and re-oxygenation

Compared to its predecessor, the CLARIOstar Plus features Enhanced Dynamic Range and Auto Focus technology, which aim to eliminate the need for manual adjustment and make detection optimization easier than before. Specialized detectors (PMTs) for different measurement methods offer additional flexibility.

The CLARIOstar Plus combines the sensitivity of filters and the flexibility of monochromators in a revolutionary new technology: our patented LVF Monochromator.

Three technologies guarantee reliable results with every measuring method:

LVF monochromators combine flexibility and sensitivity for fluorescence intensity and luminescence measurements
Filters ensure excellent sensitivity for all measurement methods (excluding absorption)
The spectrometer records entire absorption spectra in a very short time

The ClarioStar Plus can perform modern cell/molecular/high throughput assays, including:

DNA,RNA and Protein quantification
Cell based assay / Long Term Kinetic Live Cell Assays
Enzyme and Kinetic assays
Genotyping & Reporter gene assays
Molecular binding assays
ELSIA
Fluorescence Polarization
Bioluminescent Resonance Energy Transfer (BRET)
Time Resolved Fluorescence (TRF)

Sample Input:

Sample preparations in 6-384 well plates
The CLARIOstar Plus is equipped with Dual Reagent Dispensers (Dead volume 600ml)

Light Sources:

High energy xenon flash lamp
Dedicated Laser for Alphascreen / AlphaLISA / Alphaplex

Detection Modes:

Measurement Modes:

Top and bottom reading
Endpoint and Kinetic
Sequential multi-excitation
Sequential multi-emission
Spectral scanning (Absorbance, Luminescence, Fluorescence)
Ratiometric measurements
Well scanning

More time for your research – simple, automated, and flexible handling

The modularly expandable CLARIOstar Plus covers all the main detection technologies and is suitable even for highly demanding laboratory routines.

The monochromator lets you adjust the wavelengths and bandwidths for fluorescence and luminescence measurements quickly and easily.

Additional filters and an ultrafast UV/vis spectrometer for absorption measurements give you flexibility and speed.

The “Enhanced Dynamic Range” (EDR) feature simplifies the measurement setup and saves time.

Simply start your measurement, and the device will determine the ideal settings automatically. A single measurement covers the widest possible range of concentrations or signal intensities (8 orders of magnitude).

Perform long series of measurements with living cells feeling relaxed: thanks to the robust shaking and temperature control function, you can incubate your cells directly in the instrument. An atmospheric control unit (ACU) ensures the optimal composition of gases for each cell type. The ACU also allows de- and re-oxygenation, thus suitable for in vitro models of diseases such as ischemia/reperfusion.

Specification

Wavelength Range:
240 to 740 nm or 240 – 900 (red shifted PMT) for FI, FP, FRET, TRF, TR-FRET (filters),
240 to 740 nm for LUM (filters),
320 to 740 nm or 320 – 840 nm (red shifted PMT) for FI, FRET, TRF (monochromators),
240 to 740 nm for LUM (filters),
320 to 740 nm for LUM (monochromator),
220 to 1000 nm for ABS.
Optical System:
High energy xenon flash lamp,
Dedicated Laser for AlphaScreen® / AlphaLISA®,
Filters: Excitation and emission slides for 4 filters each,
LVF Monochromator™ : Excitation and Emission.
Detector(s): Low noise photomultiplier tube CCD spectrometer.
Light Source: High energy xenon flash lamp.
Measuring Time: Reading Times (Flying Mode): 9 seconds (96 wells), 14 seconds (384 wells), 27 seconds (1536 wells).
Plate Formats: 6 to 1536-well plates, LVis Plate with sixteen 2 µL microspots.
Detection System: DetectionFluorescence Intensity – including FRET, Fluorescence Polarization / Anisotropy, AlphaScreen® / AlphaLISA®, Luminescence (flash and glow) – including BRET, Time-Resolved Fluorescence – including TR-FRET, UV/Vis Absorbance Spectra

Include:

Installation and training BMG Labtech for CLARIOstar include installation and 1-day training for up to 4 users.
Bar code reader BMG Labtech for stacker III
Calibration BMG Labtech of EVPlate (681-101) with certification (recommended every two years)
EV Performance test plate BMG Labtech multimode validation plate. As listed under CLARIOstar
H3 Spoon BMG Labtech

Reference Library

CLARIOstar^® Microplate Reader Specifications

Optional Accessories
Detection Modes	Fluorescence Intensity FRET Fluorescence Polarization AlphaScreen^®/AlphaLISA^®/AlphaPlex^TM Luminescence(flash and glow) BRET Time-Resolved Fluorescence - including TR-FRET UV/Vis absorbance spectra
Measurement Modes	Top and bottom reading Endpoint and kinetic measurements Sequential multi-excitation measurements Sequential multi-emission measurements Spectral scanning (fluorescence, luminescence, absorbance) Ratiometric measurements Well scanning
Microplate Formats	6- to 1536-well plates, user-definable LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier	Robot compatible
Light Sources	High energy xenon flash lamp Dedicated laser for AlphaScreen^®/AlphaLISA^®/AlphaPlex^TM
Detectors	Low noise photomultiplier tube CCD spectrometer
Wavelength Selectiondual	Dual Linear Variable Filter (LVF) Monochromators™Linear Variable Dichroic Mirror Separates Ex & Em LVF Monochromators Optical filters: Ex and Em slides hold 4 filters each LVF Monochromators + optical filters Use one for Ex and the other for Em UV/Vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Optical Filters	Excitation and emission slides for 4 filters each
Optical Path Guides	Top and bottom: Enclosed, free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment	Automatic focal height adjustment (0.1 mm resolution)
Spectral Range	Filters	240 - 750 nm or 240 - 900 nm for FI, FP, TRF 240 - 750 nm for LUM
	LVF Monochromators™	320 - 850 nm for FI 320 - 750 nm for LUM
	Linear Variable Dichroic	340 - 740 nm for FI, LUM
	Spectrometer	220 - 1000 nm for ABS
Sensitivity*	FI Filters (top)	< 0.15 pM (< 3 amol/well Fluorescein, 384sv, 20 μL)
	FI Filters (bottom)	< 1.0 pM (< 50 amol/well Fluorescein, 384g, 50 μL)
	FI Monochromator (top)	< 0.35 pM (< 7 amol/well Fluorescein, 384sv, 20 μL)
	FI Monochromator (bottom)	< 3.0 pM (< 150 amol/well Fluorescein, 384g, 50 μL)
	FP	< 0.5 mP SD at 1 nM Fluorescein (384sv, 20 μL)
	HTRF^® (black and white microplates)	Reader Control Kit (Eu) after 18h (384sv, 20 μL) Delta F > 880 % (High Calibrator) Delta F > 30 % (Low Calibrator)
	TRF	< 20 fM Europium, 384, 80 μL
	LUM	< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL) Dynamic Range: 9 decades
	AlphaScreen^® with Laser	< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
	ABS with Spectrometer	Full spectrum captured in < 1 s / well Selectable spectral resolution: 1, 2, 5, and 10 nm OD range: 0 - 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Read Times	Flying mode (1 flash)	8 s (96), 15 s (384), 28 s (1536)
Read Times	10 flashes	19 s (96), 57 s (384), 3 min 4 s (1536)
Reagent Injection	Up to 2 built-in reagent injectors Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL) Variable injection speed up to 420 µL / s Reagent back flushing
Shaking	Linear, orbital, and double-orbital with user-definable time and speed
Incubation	+3 °C above ambient up to 45 °C or 65 °CThe upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software	Integrated fluorophore library Multi-user Reader Control and MARS Data Analysis Software included FDA 21 CFR Part 11 compliant
Dimensions	Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg
LVis Plate	Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples.Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracyDimensions: Conforms to SBS standards for microplates.
Atmospheric Control Unit (ACU)	Actively regulates O2 and CO2 - 0.1-20%
Stacker	Magazines for up to 50 plates - continuous loading feature
THERMOstar	Microplate Incubator and Shaker
Optical Filters	Excitation and emission slides for 4 filters each
Upgrades	Please contact your local DSS representative for upgrades including options such as detection modes, reagent injectors, etc.
*	LOD = 3 x SD (20 blanks) / slope (6 pt std curve) AlphaScreen^® P-Tyr-100 assay kit, PerkinElmer, #6760620C Microplates: White for LUM, AlphaScreen^®, TRF Black for FI, FP Clear for ABS 96 = 96-well microplates 384sv = 384-well small volume microplates 384g = 384-well glass bottom microplates 1536 = 1536-well microplates