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Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader

  • capable of reading both fluorescence and absorbance signals while supporting multiple wavelength options.
  • offers excellent sensitivity across all wavelengths from UV to near infrared (NIR)
  • provides a comprehensive solution for scientists looking for high throughput screening capabilities.

Original price was: $29,999.00.Current price is: $19,999.00.

SKU:SpectraMax i3x

SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. The cooled PMT detector allows high sensitivity in a wide dynamic range, even in extremely low light.

“SpectraMax plate reader is very easy to use, with detection of absorbance, fluorescence, luminescence, etc.. For prep samples treated with the ELISA kit reagents, it is fast and user friendly. The equipment has integrated software that’s also very easy to use, flexible settings and the ability to create protocols and templates for specific assay parameters.”

Two fluorescence detection channels allow the instrument to analyze cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.

The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate reader on the market.


SpectraMax i3x Features

  • User-configurable applicationsUser-configurable detection modules expand the reader’s detection capabilities to include AlphaScreen, Time-Resolved Fluorescence, HTRF, fast kinetics with injectors, western blot detection, and more.
  • No-stain cell analysisThe StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell counting workflow.
  • Multi-channel functionalityTwo fluorescence detection channels on the MiniMax cytometer enable the analysis of cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.
  • Cellular pathway analysisCell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein can be captured on one reader.


Add Cellular Imaging to your Plate Reader Workflow

Observe your cells’ viability with the field-upgradeable cellular imaging option for the SpectraMax i3x Multi-mode Detection Platform.

The field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes accompanying cytotoxicity, cell proliferation, and protein expression. Now with StainFree™ Cell counting Technology, additional brightfield analysis features, and fluorescent green and red detection channels.

Assure data integrity and compliance with confidence

Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.

  • Best-in-class microplate readers and washers support all your assay needs
  • IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
  • Software installation services verify and document that required components are installed to operational speci?cations
  • Software validation service supports FDA 21 CFR Part 11 guidelines
  • Validation plates test the performance of your microplate reader using traceable materials for reliable results

SpectraMax i3x Advantages

  • The instrument can be upgraded with fluorescence polarization, delayed fluorescence (TRF), HTRF®, AlphaScreen® and more with application cartridges – installation in minutes
  • The only reader enabling the detection of western blots on membranes
  • Option to expand the system with a SpectraMax® MiniMax™ Imaging Cytometer for visualization of cell morphology
  • Spectral Fusion™ Illumination – a unique type of light source for an ultra-strong signal with increased fluorescence performance
  • Dual injectors with a smart injection function and the possibility of reverse liquid suction can be purchased for the device
  • Extended dynamic range with optimal sensitivity thanks to a cooled photomultiplier
  • SoftMax® Pro software for unique, comprehensive data analysis is part of the basic equipment

Applications of SpectraMax i3x Multi-Mode Microplate Reader

  • Absorbance
  • AlphaScreen
  • Cell Health
  • Cell Imaging > Cell Cycle Analysis
  • Cell Imaging > Cell Migration
  • Cellular Signaling
  • Fluorescence
  • Fluorescence Polarization (FP)
  • Kinase/Phosphatase Assays
  • Luminescence
  • Microbiology and Contaminant
  • Nanoparticles
  • Nucleic Acid (DNA/RNA) Quantitation and Analysis
  • Protein Detection, Quantitation, and Analysis
  • Spheroids in 3D biology
  • Western blotting


  • SpectraMax® i3x Multi-mode detection platform
  • SpectraMax™ injector cartridge
  • Bottle holder for injector cartridge
  • Waste plate for injector cartridge
  • Tubing for injector cartridge
  • Adaptor for injector cartridge


General specifications

Read modes
Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read) Fluorescence Polarization (top read) HTRF (top read) AlphaScreen (top read)
Wavelength ranges
Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm
Wavelength selection
Cartridge filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity
< ±0.010 OD ±1.0%, 0 to 2 OD
Absorbance photometric precision/repeatability
< ±0.003 OD ±1.0%, 0 to 2 OD
Sensitivity top read optimized
FL: Mono: 0.5 pM fluorescein 96 well, 1 pM fluorescein 384 well; Cartridge: 0.1 fmol/well in 75 μL fluorescein 384 well, 0.03 fmol/well in 8 μL fluorescein 1536 well Lumi: Mono: 3 pM ATP-Glow 96 well, 6 pM ATP-Glow 384 well; Cartridge: 20 amol ATP-flash TRF cartridge: 3 amol/well in 100 μL europium (0.03 pM) 384 well, 1 amol/well in 8 μL europium (0.125 pM) 1535 well FP cartridge: 3 mP (75 μl) fluorescein 384 well, 6 mP (8μl) fluorescein 1536 well AlphaScreen cartridge: <100 amol/well 384 well
Light source(s)
High power xenon flash lamp LEDs in visible range
Silicon photodiode Cooled photomultiplier tube
Read types (endpoint, kinetic, etc)
Endpoint Kinetic Spectrum scan Well scan
Read times
Abs: 29 seconds FL: 22 seconds Lumi: 21 seconds TRF: 21 seconds FP: 38 seconds AS: 22 seconds
Plate shaking
Linear Orbital
Western blot
Robotics / automation
Plate type(s)
6-1536 well plates
Temperature control
Ambient + 4°C to 45°C
H 32.5 cm x W 39.2 cm x D 60.55 cm
Validation tools
Absorbance validation plate Fluorescence validation plate Luminescence validation plate Multi-mode validation plate SoftMax® Pro GxP Software


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