BioTek Synergy H1 is a multi-modality microplate reader configurable with monochromator-based optics for flexibility,  filter-based optics for sensitivity, or both. BioTek’s patented Hybrid Technology offers versatility and high performance in a capable modular platform to expand as your laboratory needs change.

BioTek Synergy H1 now offers variable bandwidth fluorescence monochromators for excitation and emission wavelength selection; Fluorescence bandwidth can be adjusted between 9nm and 50nm in 1nm increments, allowing users to fully optimize reader settings for best assay performance compared to wideband systems.

BioTek Synergy H1 features:

• Absorbance, fluorescence, and luminescence can be measured with a single unit.
• Supports 6, 12, 24, 48, 96, and 384 wells
• Absorbance can be set between 230-999nm (in 1nm steps)
• Fluorescence wavelength can be set between 250-700nm (900nm is optional)
• Temperature setting: room temperature to 45 ° C (4-zone temperature control method – excellent temperature uniformity )
• Shaking: Linear, Orbital, Double-orbital ( mode suitable for cell experiments )
• Gen5 software: Sample measurement and analysis quickly and easily
• Excellent durability, compact size

Hybrid plate reader: Flexibility and performance

Synergy H1 is an advanced plate reader that delivers both the flexibility and performance you need for any microplate assay in your lab with its patented combination of monochromator and filter optics.

Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence

Upgradable to meet future application needs

Synergy H1’s modular design allows you to start with what you need now and add detection modes, gas control, and dual reagent injectors as your laboratory’s workflows evolve.

Variable bandwidth for sensitivity and specificity

Synergy H1 offers quad monochromator optics with variable bandwidth. The bandwidth can be set between 9 and 50 nm in 1 nm increments. Large bandwidths (1) provide increased sensitivity and lower limits of detection. Small bandwidths (2) provide increased specificity when multiple signals are present, reducing crosstalk and enhancing assay performance.

Automated z-focus: best performance with all plate types

(1) Without automated z-focus available, performance at low volumes is affected. (2) With automated z-focus, reading height is precisely adjusted for the best performance in all plate types and all volumes.

Extended dynamic range

Synergy H1 offers an extended dynamic range, which allows the detection of signals across a 7-log measurement range. Other systems can measure only small portions of the dynamic range of Synergy H1 using preset gains – this can cause reduced sensitivity on the low end or saturated signals on the high end of the assay signal range.

Environmental controls for cell-based assays

Temperature control to 70 °C, CO2/O2 control, condensation control and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays.

Dual syringe injectors with specialized tips

1) The robust, precise dual syringe design eliminates the need for regular tubing replacement required by some peristaltic injector designs. Synergy H1 offers two tip types: (2) the straight tips enable vigorous mixing for rapid inject/to read assays, and (3) the angled tip option won’t disturb cell layers for applications such as calcium kinetics.

Micro-volume analysis with Take3 plate

Enable micro-volume analysis with the Synergy H1 using the Take3 Plate. Measure up to 16 or 48 samples in one run and save a lot of time compared to single-sample devices. Gen5 has customizable protocols for ssDNA, dsDNA, RNA, and protein quantification in 2 µL.

Application Areas:

• Nucleic acid quantification
• Protein quantification
• Enzyme kinetics
• Biomarker quantification
• ELISAs
• Yeast kinetic analysis
• Genetic analysis
• Drug discovery
• Cell proliferation
• Cytotoxicity
• Drug absorption and metabolism
• Biologics drug discovery and development
• Food safety
• Environmental monitoring

Synergy H1 includes Gen5 Data Analysis Software for advanced reader control, data analysis, graphing, exporting, and reporting. Additionally, Synergy H1 is compatible with BioTek’s Take3 Multi-Volume Plate that measures up to 16 2 µL samples, two BioCells, or a standard cuvette for fast and simple multivolume, multisample analysis.

The modular architecture means users can easily upgrade from a monochromator-based multimode reader to a hybrid reader, offering versatility and unlimited assay use.

Reference Link

• Manufacturer Information
• Brochure