Thermo Scientific Multiskan SkyHigh Microplate Spectrophotometer

Multiskan SkyHigh Microplate Spectrophotometer provides many benefits to clinical and research labs. The Multiskan SkyHigh Microplate Spectrophotometer features an automatic wavelength calibration that compensates for environmental fluctuations in temperature, light intensity, vibration, or other factors that could produce inaccurate readings. Additionally, advanced optics allow for greater detail in measurements than traditional optical instruments.

“As far as simplicity goes, Multiskan SkyHigh spectrophotometer is hard to beat. For day-to-day use with or without a connected pc compared to our old spectrophotometer it excels at readout speed and assay setup. We mainly use it for endpoint cytotoxicity assay analysis and protein / DNA content analysis (thanks to a handy μDrop plate). High readout accuracy is expected from a modern spectrophotometer and in this department, it also does not disappoint. The software is also very clear and intuitive (the same as in the Varioskan lux) but for fast and simple tasks we often use the integrated LCD screen. This device is also very easy to use for beginner users, even our students can use it without an issue within 15 minutes of training.”

• Łukasz Kaźmierski – Collegium Medicum Bydgoszcz

Multiskan SkyHigh is a UV/VIS spectrophotometer for measurements in multi-well plates. The device allows you to carry out virtually all photometric measurements, but it is especially recommended for DNA, RNA, and protein measurements.

It is very easy to use and accessible to users. It is perfect in laboratories where kinetics, spectral analysis, or concentration measurements are performed. Multiskan SkyHigh cameras are available in 3 different configurations. The option with a touch screen allows flexibility in use – the camera can work without connecting to a computer, or it can be operated using the SkanIt program after connecting.

There is also a version without a touch screen and an additional cuvette module. Furthermore, its robust design makes it capable of enduring harsh working environments with minimal maintenance needs.

Multiskan SkyHigh Features:

• Monochromator-based optical system for free selection of wavelengths from 200 nm to 1000 nm
• Compatible with 6-48, 96, and 384-well microplates or cuvettes with a maximum plate height of 19.5 mm, including possible lid
• Reads µDrop™ and µDrop Duo plates for DNA/RNA and protein microvolume analysis
• Separate optimized measurement modes for absorption and turbidimetric measurements
• Enables kinetic, spectral, and endpoint measurements for a variety of applications.
• Fast operation: the complete spectrum of a well is performed in less than 10 seconds; The typical DNA spectrum can be measured in less than three seconds, and an entire 96-well microplate is read at one wavelength in 6 seconds.
• Performs onboard shaking and incubation for cell-based or critical temperature assays
• Touchscreen models have a user-friendly interface for stand-alone use. They include ready-to-use protocols for UV-based protein, nucleic acid quantification, and colorimetric protein quantification.
• Thermo Fisher Connect or Microsoft OneDrive cloud-based tools allow you to store securely, access, share and manage your photometric data remotely (touchscreen models)
• Multiple languages ​​available; choose from English, German, French, Spanish, Italian, Portuguese, Russian, Chinese, and Japanese (touchscreen models)
• The most demanding assay setup is made easy through the powerful and intuitive SkanIt software.
• Multi-instrument software platform compatible with all Thermo Scientific microplate readers
• Open license software for unlimited installation on multiple computers
• Research Edition for the Life Sciences; Drug Discovery Edition with features to comply with FDA 21 CFR Part 11
• Available user interface languages: English, French, German, Spanish

Supports photometric measurements depending on version and retrofit:

• in 96- and 384-well microplates;
• in cuvettes with optical path lengths up to 10 mm;
• in micro volumes of 2-10 µl using the µDrop Plate.

Measuring modes:

• optical density (simple and differential measurement, blank correction);
• kinetic mode with the measurement at one wavelength or with spectrum scanning;
• calculation of concentration by a known conversion factor or by a known calibration curve (4 types of the curve);
• determination of DNA/RNA concentration;
• removal of the spectrum of the sample;
• a qualitative classification of samples (below the norm / normal / above the norm).

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